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The number after the x tells you how many lanes (how data travels to and from the PCIe card) that PCIe slot has. A PCIe x1 slot has one lane and can move data at one bit per cycle.

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If you purchase a PCIe 4.0 GPU for your system and seek to benefit from PCIe Gen 4's performance increases and reduced latency, then your motherboard will need to be populated with a PCIe 4.0 slot of appropriate lane size to support your PCIe 4.0 GPU.